The Globe Cinema at Portage Place Mall will show its final film Sunday, June 15.
Movie-goers are greeted by a notice at the box office that blames declining attendance numbers for the closure.
The theatre has been owned by Calgary-based Landmark Cinemas for more than a decade.
"We have always been committed to our movie going guests in Winnipeg and therefore we remained in this location while trying a variety of programs to make this theatre viable," said Landmark COO Neil Campbell. "Recently, it has become increasingly difficult to get films for this location due to the low attendance numbers, therefore, it was time to make the hard decision to close."
Landmark Cinemas Canada also operates Towne Cinema 8 in the Exchange District and Grant Park Cinemas.
The company said gift cards and vouchers from Globe Cinema will be honoured at those locations.
